People are trying to help you out here. It is rather well known that you need to use calibration solution, not RO water, to calibrate your refractometer. This has been demonstrated several times, the reasonings of which are listed in a very informative thread on here as well. Unfortunately the myth persists that you can calibrate using RO water - the instructions that came with my refractometer even said as much! They were wrong, however. This is easily demonstrated by getting some solution that is specifically made to be an exact 35ppt, and just testing it. For some meters it works out to be close, for others it's as far off as 1-2 points or more. Plus, refractometer calibration solution is cheap - under $5.. If nothing else it's worth the peace of mind. Although, as has been mentioned, it's your tank, you can used and do what you want.
Regardless, I hope you find the source of the disappearing salinity soon.